Extended field-of-view light-sheet microscopy

Light-sheet fluorescence microscopy enables rapid 3D imaging of biological samples. Unlike confocal and two-photon microscopes, a light-sheet microscope illuminates the focal plane with an objective orthogonal to detection axis images it in single snapshot. Its combination high contrast minimal sample exposure make ideal image thick samples sub-cellular resolution. To uniformly illuminate wide field-of-view without compromising axial resolution, propagation-invariant light-fields such as Bessel Airy beams have been put forward. These do however irradiate relatively broad transversal structure. The excited by side lobes can be blocked physically during recording; though at cost increased exposure. In contrast, beam has fine structure that is both curved asymmetric. captures all high-frequency components enable resolution need discard useful fluorescence. This advantage does not carry over naturally excitation where suppressed. We demonstrate symmetric planar used rely on deconvolution.